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Confocal Microscopy
by - 26 February 2020 (modifié le 2 June 2021)
TCS SP5 AOBS LEICA Microscope , equipped with UV laser and visible lasers covering the spectral range from 351 to 633 nm. It’s also coupled to a pulsed IR Ti: Sa laser for two-photon imaging.
Three photomultipliers (PMTs) are available for multi-color fluorescence intensity imaging and for spectral imaging. A FRAP license is also made available to users.
For Fluorescence Lifetime Iimaging : FLIM), the microscope is coupled to a IR Ti: Sa pulsed laser (680-1040 nm) from Coherent, and to an avalanche photodiodeconnected with a PicoHarp 3000 (PicoQuant)for time correlated single photon counting measurements (TCSPC).
It is also possible to produce FLIM spectrally resolved images thanks to a dedicated fast photomultiplier within the confocal microscope.
The PicoHarp device also allows fluorescence correlation measurements ( FCS fluorescence correlation spectroscopy ) under 1 or 2 photon laser excitation.
The confocal microscope is equipped with a thermostatically controlled and CO2-regulated enclosure which may be necessary for experiments carried out on eukaryotic cells or living bacteria.
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