Mechanistic and Structural Characterization of Organometallic and Biochemical Systems using Advanced Mass Spectrometry
Lyna Bourehil
Department of Chemistry and Applied Biosciences, ETH Zurich
Vendredi 23 mai à 11h – ISMO Amphitheater
My research focuses on the development and application of advanced analytical methods. Here, mass spectrometry and spectroscopy are used to characterize the physicochemical properties of target compounds and elucidate their reaction mechanisms. During my PhD, I investigated the electronic effects of ligands on organometallic complexes and their influence on catalytic activity. Mass spectrometry, photoelectron spectroscopy, and RRKM statistical modeling were used to develop a direct method for measuring metal–ligand bond dissociation energies. The resulting data provided insights into how ligand substitution modulates the electronic enrichment of the metal center and thus its reactivity.1,2 As a postdoctoral researcher, I explored peptide fragmentation mechanisms using multiple activation techniques, kinetic modeling, and molecular dynamics simulations, revealing unexpected fragment ions and clarifying their formation pathways.3 My current postdoctoral work focuses on probing non-covalent interactions in biochemical systems using native mass spectrometry, cyclic ion mobility, and multiple fragmentation methods to uncover thermodynamic and kinetic parameters of biologically relevant compounds.
References
1. L. Bourehil, C. Soep, S. Seng, S. Dutrannoy, S. Igoudjil, J. Forté, G. Gontard, D. Lesage, B. Bertrand, H. Dossmann; Inorg. Chem., 62, 13304–13314 (2023).
2. L. Bourehil, N. Joly, L. Bettoni, J. L. Renaud, D. Lesage, H. Dossmann, G. Garcia; Inorg. Chem., (to be submitted).
3. L. Bourehil, Q. Duez, R. Kamguem Kamga, M. Devies, A. Walrant, L. Matheron, P. Gerbaux, D. Lesage, J. De Winter, E. Sachon; (to be submitted).